Genetic variation knowledge is critical in muskmelon crop improvement. For genetic diversity evaluation in twenty-five muskmelon genotypes twenty microsatellite markers were used in the present study. Nineteen SSRs were polymorphic and reproducible among twenty. The expected heterozygozygosity (He) values varied from 0.39 to 0.46 at an average of 0.42, the observed heterozygozygosity (Ho) was 0.31 to 0.59 with a mean of 0.49 and these values were somewhat more than the expected heterozygosity, indicating that the population is naturally highly heterozygous. PIC values varied from 0.54 to 0.84, with a mean of 0.69. This data confirmed the polymorphism at the molecular level, indicating these genotypes make up a remarkable basis of muskmelon diversity. Cluster analysis was used to assess the relationship between genotypes and genotypes were divided into three clusters.